How to Use Liquid Culture Syringes to Inoculate Jars and Bags

Welcome to BeeShroomin’s step-by-step guide on using liquid culture syringes for inoculating your substrate jars and bags. This page will help you understand the techniques to ensure a sterile environment, which is crucial for successful mushroom cultivation.

What You’ll Need:

  • LC syringe with your chosen mushroom culture
  • Sterile substrate jars or bags
  • Alcohol wipes or isopropyl alcohol
  • Flame source (like a lighter or a propane torch)
  • Sterile gloves
  • Oven, flow hood, or still air box (SAB)

1. Preparing Your Workspace: Choose a method for creating a sterile environment:

Oven Method: Preheat your oven to a low temperature (around 200°F). The idea is to create a warm air curtain where the hot air flows out and prevents contaminants from entering your workspace. Set up your materials on the open oven door and work in this space.

Flow Hood: A flow hood provides a sterile air flow across your workspace. Set it up in a clean area, turn it on, and let it run for a few minutes before you start working. Place all your tools and materials under the hood.

Still Air Box (SAB): A simple and effective option, especially for hobbyists. A still air box is essentially a large clear box with armholes. Clean and wipe it down with alcohol before use, then place all your materials inside and work through the armholes.

2. Sterilizing Equipment:

  • Wipe down the LC syringe, needle, and the injection ports of your jars or bags with alcohol wipes.
  • Heat the needle of the syringe with a flame until it is red hot, then allow it to cool for a few seconds.

3. Inoculation:

  • Shake the syringe well to distribute the mycelium evenly.
  • Insert the needle through the injection port or into the substrate bag, being careful to keep the syringe steady.
  • Inject 2-3 ml of liquid culture into the substrate. The exact amount can vary based on the size of your substrate and the growth characteristics of the culture.
  • Carefully withdraw the needle and seal the injection port if using jars.

4. Post-Inoculation Care:

  • Place the inoculated substrates in a dark, warm area where they can colonize. Ideal temperatures and conditions vary by mushroom species, so refer to specific guidelines for best results.
  • Monitor the jars or bags for signs of mycelial growth and contamination.

Tips for Success:

  • Always work quickly and efficiently to minimize the time your substrates are exposed to potential contaminants.
  • Dispose of or sterilize your tools after each use to maintain cleanliness.

By following these steps, you can effectively use LC syringes to start your mushroom cultivation with a high chance of success. Remember, sterility is the key to avoiding contamination and achieving the best yields from your efforts.